肉鸡肌肉与脂肪组织基因组差异剪接基因分析

doi:10.11843/j.issn.0366-6964.2018.10.008

Abstract

Abstract:

The objective of this study was to identify and analyze the differential splicing gene (DSG) between muscle and fat tissues genome in AA broilers. The transcriptomic profiling of breast muscle and abdominal fat tissues (n=3) in AA broilers were sequenced using Illumina Hiseq 2500 technology. The alternative splicing event (AS) and DSG between muscle and fat tissues genome in AA broilers were identified using rMATS software. The GO function enrichment and KEGG pathway analysis were performed for DSG. The results showed that (5 966.00±111.66) and (6 757.00±156.51) (P=0.002) alternative splicing events (AS) were identified in genome of muscle and fat tissues, respectively in AA broilers. Skipped exon (SE) was the main events with the highest frequency of 54.92% and 52.67% of the total number of AS in muscle and fat tissues, respectively; A total of 513 significant DSGs were identified between muscle and fat tissues genome. The GO enrichment analysis indicated that 93 DSGs were significantly enriched in cellular component and molecular function. A total of 31 DSGs were significantly enriched in the regulation of actin cytoskeleton, focal adhesion, pyruvate metabolism and endocytosis pathways;The mRNA expression levels of 14 DSGs were not significantly different between muscle and fat tissues. Through the genome-wide identification and analysis of DSG in muscle and fat in AA broilers, the results of this study will provide insight into the mechanisms of alternative splicing and diversity of differential splicing.

CLC Number:

S831.2

ZHANG Min, WANG Jie, SUN Yan-fa, ZHONG Li-peng, LI Yan, XU Wei-hua, LI Hong-yi, ZHANG Mao, HUANG Qi-chun. Differential Splicing Gene Analysis between Muscle and Fat Tissues in Broilers[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(10): 2124-2132.

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Differential Splicing Gene Analysis between Muscle and Fat Tissues in Broilers

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